Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase
نویسندگان
چکیده
Helicase proteins are known to use the energy of ATP unwind nucleic acids and remodel protein-nucleic acid complexes. They involved in almost every aspect DNA RNA metabolisms participate numerous repair mechanisms that maintain cellular integrity. The archaeal Lhr-type SF2 helicases mostly uncharacterized. have been proposed be act recombination processes Sulfolobales Methanothermobacter. In Thermococcales, a protein annotated as an Lhr2 was found network metabolism. To investigate this, we performed in-depth phylogenomic analyses report classification taxonomic distribution Archaea, better understand their relationship with bacterial Lhr. Furthermore, goal envisioning role(s) aLhr2 Thermococcales cells, deciphered enzymatic activities from Thermococcus barophilus (Tbar). We showed Tbar-aLhr2 is DNA/RNA helicase significant annealing activity dependent on transactions.
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ژورنال
عنوان ژورنال: Biomolecules
سال: 2021
ISSN: ['2218-273X']
DOI: https://doi.org/10.3390/biom11070950